| Identifiers |
 |
| Product Name |
Czapek-Dox Broth |
| Catalogue No. |
CM510236 |
|
Description |
Czapek-Dox medium, both liquid and solid version, is a general cultivation medium of defined chemical composition, where the sole nitrogen source is sodium nitrate, and the carbon source is sucrose. It has been employed successfully in the isolation and cultivation of soil microorganisms especially fungi. Its original version had a pH near neutrality, but it can be rendered selective medium for the fungi by adding, (after sterilization and before solidification), 10 mL of sterile lactic acid 10% solution, producing a pH drop to 3,5. In this medium, Warcup adds 5 g/L of Yeast extract (Ref. 07-079) and an antibiotic mixture (Streptomycin 30 mg/L and Tetracycline 2 mg/L) to achieve total efficacy and selectivity. In any case, bacterial growth is very poor. Czapek-Dox medium has been adopted for the morphological studies of soil fungi, and it aids chlamydospora formation by Candida albicans in shorter periods (Dawson, 1962). Temperatures and times of incubation always varies, and they can |
|
Application |
Semisynthetic liquid culture medium with sodium nitrate as the only nitrogen source. |