Home

Drug development, from research via clinical trials to an approved product, is a cumbersome and expensive process. The breath-taking dynamics of the market for biopharmaceutical products does not leave any room for experiments. No mistakes are permitted. The availability and presence of experts therefore becomes indispensible.

We have set up an international team consisting of scientists, marketing specialists and experienced business people; this team focuses its activities on your specific requirements in biotechnology and the production of biopharmaceuticals.

For more information please email sales@advtechind.com

These are the building blocks of life, the substances that proteins are made of. The L-amino acids and their derivatives are key biochemical compounds in the life sciences. They are used in large quantities as raw materials, excipients and active ingredients in the pharmaceutical and chemical industries. They are also used as additives in the food and animal feed industries. In the area of biotechnology, they are essential components in practically all fermentation and cell culture processes.

The quality demanded of such amino acids for use in pharmaceutical applications is prescribed by the procedures contained in the most commonly used pharmacopoeias (DAB, Ph.Eur, USP etc.). In addition, when used in biotechnological applications, particularly for the manufacture of pharmaceutical active ingredients, further requirements have to be fulfilled with respect to product properties.

Our stringent analytical control procedures guarantee high and uniform quality and excellent batch-to-batch consistency

Our comprehensive range of extra pure amino acids has now been further upgraded: As from now, all L-amino acids of the "for biochemistry" grade can be optionally provided according to DAB, Ph.Eur and USP specifications and indicated accordingly on the Certificate of Analysis. This provides additional reliability and facilitates upscaling from laboratory to production scale.

The following table provides an overview of the L-amino acids provided and in accordance with DAB, Ph.Eur and USP specifications:

For more information on Amino Acids from Advanced please email sales@advtechind.com

Fractogel EMD media enable all those involved in bio-pharmaceutical processing the use of a well tested production tool. Compared to conventional resins, Fractogel EMD media improve the efficiency of the separation part of the production process. Yields are higher, processes can be conducted at higher flow rates therefore reducing throughput time. Because the media last so long and can be used repeatedly, the economics of the process are very favourable.

The structure of the Fractogel® particles is considerably different from that of other hydrophilic chromatographic resins like dextran, agarose or cellulose.

Fractogel® is a synthetic methacrylate based polymeric resin providing an excellent pressure stability resulting in high flow rates. The process media consist of beads with a particle size between 40 and 90 mm. Fractogel® EMD BioSEC for size exclusion chromatography has a particle size in the range 20-40 mm. The pores which are formed from intertwined polymer agglomerates, have a size of about 800 Å enabling a free diffusion of proteins into the beads. The complete surface is strongly hydrophilic due to the ether linkages in the polymer.

Long, linear polymer chains ("tentacles") carry the functional ligands. All tentacles are covalently attached to hydroxyl groups of the backbone structure of Fractogel®. Thus, both the bead and surface modification are stable to regeneration and sanitisation. The main advantage of the tentacle chemistry is the large amount of sterically accessible ligands for the binding of biomolecules without any steric hindrance. Target biomolecules are, therefore, much more tightly bound during the separation process. Different ligands are utilised for various application areas (ion exchange, affinity, hydrophobic interaction chromatography).

Work involving biological molecules invariably involves the use of buffer solutions. The task of the buffer is to create a defined environment for the biomolecule so that it can be kept in a stable and native form so that its activity is not influenced. The buffer itself, however, should not exert any influence on the biological compound. Equally important, the buffer should not contain any impurities that may have a negative effect on the activity or stability of the biomolecule. Hence, only highly purified substances of defined specification should be used which guarantee reproducible processes on a lab or production scale.

VWR International supplies buffers in various grades. The differences between them rarely involve purity itself but more the additional parameters that are checked prior to delivery. For example, whilst the content of nucleases may be an important quality criterion for biotechnological applications, the fulfilling of the requirements of a particular pharmacopoeia may well be critical when it comes to manufacturing a pharmaceutical active ingredient. However, for certain applications, it may be desirable to include additional quality parameters not included in our Certificate of Analysis in the testing programme. Normally it is possible for this to be done so that a customised product results.

The same applies to individual package sizes. You may wish to have a pack containing precisely 11.4kg of sodium chloride for frequent routine use. We could supply this as a special pack size. Alternatively you may require a special mixture of buffers, again in most cases we can oblige. We can produce mixtures, granulates, ready-to-use solutions and concentrates according to your wishes or using your procedures – all of course in strict confidentiality.

For more information on buffers for bioprocessing from Advanced please email sales@advtechind.com.

Require another pack size?
Ask us. Special packaging is almost always possible.

Other special packaging arrangments?
Ask us. We will suggest alternatives.

Require additional test parameters on the Certificate of Analysis?
Let us know. We will gladly advise you.

Require customised mixtures?
Ask us for a quotation.

For more information please email sales@advtechind.com

Benzonase^® is a unique genetically engineered endonuclease offering a variety of advantages over existing methods of nucleic acid removal.

Is ideal for a variety of applications including:

Benzonase^® ELISA II assay kit was developed for the detection and quantitative determination of Benzonase^® in samples from downstream processing where Benzonase^® is used as a process or purification aid.

For more information on Benzonase please email sales@advtechind.com.

Make the preparative HPLC columns you want, when you want and be sure the quality is excellent.

"Selfpacker" preparative columns are:

It's clear! For quality results you need a quality column. However, you also know that the range of quality preparative columns is limited and the costs are very high. But why be limited by the selection of stationary phases available in pre-packed preparative columns? Why pay the high costs?

With the "selfpacker" packing stand technology, developed through close collaboration with both customers and engineers, you can be assured of high quality semi-preparative and preparative HPLC columns, the flexibility to respond quickly to changing needs and significant cost savings.

Benefits at a glance:

Simple: The new vacuum packing technology permits simple creation of preparative columns.

Quality: Due to the stabilisation of the column bed, packed columns can be removed from the stand and are fully operational. Columns with temperature control jackets provide optimum separation and performance, with excellent reproducibility.

Versatile: Even soft gels such as polymer based gels can be packed with the new packing devices. Columns can be easily emptied and rapidly refilled with a new sorbent of choice. Customised column lengths can be provided on request.

Fast: The separation columns can be prepared rapidly on-site.

Economic: The fully controlled gel bed compression leads to a longer lifetime of the columns and improved process economy. One single packing stand can be used to pack an unlimited number of columns with the same inner diameter.

"Selfpacker" packing stands are available with 25, 50 and 100mm I.D. Other options include; a chain bolted flange version which has proven particularly useful for the 100mm I.D. column where the stationary phase has to be changed regularly and; a jacketed version for temperature control.

top

Standardised silica gels - The basis for reproducible chromatography from the laboratory to production.

Merck has specialised over many decades in the manufacture of standardised silica gel packing materials for preparative chromatography and offers a wide range of customised sorbents. Such gels provide the user with unique method reliability. Regardless of which chromatography process is used, direct and rapid transfer to a production scale is guaranteed and without risk. This is ensured by the fact that identical raw materials are used for all stationary phases, thus providing identical chromatographic selectivity.

In order to be able to achieve this goal, Merck produces all silica gel 60 packing materials for analysis, pilot plant processes and production using the same silica gel raw material which has specified surface area and a defined specific pore volume. The selectivity of Merck's silica gels for analytical and preparative applications is identical.

Merck guarantees direct scale-up without compromise and without having to lose time. Quality guarantees from Merck have been the standard for guaranteed quality in analysis for over 100 years.

Two examples of Merck products are, LiChroprep® an irregular silica gel packing material and LiChrospher® for preparative high-performance chromatography a spherical and porous silica gel material. Both materials are characterised by:

It is quite possible that certain separations can only be carried out using silica gels that have been pre-treated in a certain way. Should you require for example, acid-washed silica gels in certain particle size ranges or in any other pre-treated form, please contact us. Special production batches also present no problems for us.