•

Air Sampling

•

Culture Media & Supplement

•

Gas Generating Kits

•

Rapid Identification Kits

•

Stains &
Consumable
Apparatus

Air Sampling

MAS 100 Eco NEW

The new MAS 100 Eco is the latest addition to our line of air samplers and offers an economical alternative to our highly acclaimed MAS 100 sampler. Designed specifically with the food and beverage industry in mind, the unit offers an easy, low cost method for monitoring.

The lightweight unit uses standard 100 mm petri dishes which means, you can use the same media that you use in your lab. No revalidation is necessary. The MAS 100 Eco uses the impaction method, approved worldwide, as do all of our other samplers. The handle can also be used as a stand, to allow different angles for testing. The perforated lids are interchangeable with our other aluminium models. Internal software is easily accessed and controlled using single touch "yes" or "no" responses.

Technical specifications include an airflow of 100 litres/minute. The unit and lid cover are both made of anodized aluminium and the lids are autoclavable. Power is supplied via NiMH rechargeable batteries. Run time per charge is 3 hours and maximum time for complete recharge is 9 hours. Charger is supplied. The display is alpha numeric LCD, 16 digits with real time and date. There are 6 preset volumes: 10, 20, 50, 100, 200 and 500 litres. Users can also reset any of the presets to any volume between 0-1000 litres.

MAS 100

In many industries, especially in the pharmaceutical and food industries, the requirements for monitoring and documentation of the air quality become more and more stringent. The contamination of the air with micro-organisms can now be monitored more easily, precisely and inexpensively with the MAS 100 Air Sampler from Merck Eurolab.

This handy air sampler is ideal for use with standard 90mm petri dishes and an integrated anemometer ensures correct and reproducible results. It is light, portable and easy to use and the adjustable sampling head allows any measurement from horizontal to vertical airflow.

Based on the principle of the Anderson Air Sampler, the MAS 100 air sampler meets the requirements of the "Regulations for medicines and the European Community" (volume IV).
top

MAS 100Ex - Explosion Proof

Merck Eurolab has now introduced the MAS 100Ex air sampler which combines all the well established advantages of the MAS 100 but can be used for the microbiological monitoring of the air in explosion proof zones.

Technical specifications include an airflow of 100 litres/minute, programmable start-delay function of up to 60 minutes, pre-programmed collection volumes of 10, 20, 50, 100, 200, 250, 500, 750 and 1.000 litres, real time and date, factory calibrated with certificate and an exchangable battery pack for remote operation of up to 7 hours.

The unit is constructed according to the safety requirements specified in the norm DIN/VDE 0165 "Installation of electrical apparatus in hazardous areas (Elex V)" and tested and certified according to prEN 5021 "Specification for electrical apparatus with type of protection ‘n’ (non sparking)."

MAS 100ISO

The MAS 100ISO is an innovative concept for the efficient monitoring of the microbiological cleanliness in isolators and clean-rooms. The sampling head(s) can be fixed in all locations to be controlled without having any electronic or mechanical components in this control zone. A specially developed double-valve system allows the unconditional integration of the sampling head(s) into the complete sterilization process of the isolator. Each control unit can run 4 sampling heads and in a BUS system up to 40 sampling heads at the same time, by means of starting, monitoring and controlling. The sampling method used is based on the well-known and proven impaction principle developed by ANDERSEN.

Technical specifications include: an airflow of 100 litres/minute, aluminium or stainless steel sampling head and perforated lid, integrated printer and anemometer, compliant with RS232/48S connector for data transfer, remote control start and in-line sterilisation.

For more information on air samplers available from Advanced please email sales@advtechind.com  

 top

Culture Media & Supplement

Granulated Media

Merck Eurolab's range of tailormade media can overcome many of the traditional problems experienced with powdered culture media because of our unique granulation technique. Components are subjected to our special process which binds the powder to form small solid granules. This reduces or eliminates problems such as media clumping in the bottle, poor solubility, component separation and dust contamination. The smaller proportion of dust reduces inhalation of toxic substances, allergic responses and contamination of laboratory equipment and clothing. The end result is a safer environment and more reliable analytical results.

Chromogenic Media

Merck Eurolab's range of chromogenic media includes Chromocult Agar, which allows simultaneous detection of and differentiation between total coliforms and E. coli. It produces characteristic colony colours to aid the identification of bacteria, reduces errors and improves accuracy, saving time, labour and cost. Also in the range is Chromcult Enterococci Broth, a new medium specifically designed to detect Enterococci in water with accurate, targeted and rapid (18-48 hours) results.
top

Fluorogenic Media

Fluorocult is a full range of fluorogenic culture media and equipment which enables effective, rapid and reliable detection of E. coli coliforms in water and food. Fluorocult is ideal for use in dairy, meat, water, pharmaceutical, cosmetic and clinical diagnoses.

Irradiated Media

Merck Eurolab also has a range of media, including Tryptic Soy Broth. The medium is very suitable for the simultation of aseptic filling of sterile powder and liquids. This allows the cultivation of even fastidious micro-organisms. It's a soluble powder which is diluted in sterile water, falls in line with stringent health and safety guidelines and is both user freindly and economic.

E. coli 0157 Range

The Veratoxin-forming strains of E.coli human pathogens have gained importance recently, particularly the group of Enterohemorrhagic E. coli with its highly pathogenic serovar 0157.

Merck Eurolab has a comprehensive range of products for the enrichment, isolation and detection of this micro-organism which includes mEC broth, mTSB, Flurocult HC agar, SAC agar with the necessary selective supplements, Novobiocin and CT-supplement.

The culture media comply with USDA-FSIS and DIN 10167 for its detection in foods and FDA-BAM for the isolation of EHEC.

For more information please email msgroup@merckeurolab.ltd.uk or call our literature request line on 01202 664421.

Fields of Applications:

top

Anaerocult Gas Generating Systems

The importance of anerobic and micro-aerophilic micro-organisms as agents causing infectious diseases or the spoilage of food, calls for a reliable system to facilitate the removal of oxygen by the production of carbon dioxide. Anerocult is a complete system which does not require a catalyst and which meets this demand.

top

Rapid Identification Kits

LATEX AGGLUTINATION TESTS

Merck Eurolab offers a range of Rapid Microbial Identification products for the food, pharma and environmental market place.

The Microgen range includes several latex test kits for rapid identification of:

Merck Eurolab also offers additional identification products including:

BACTIDENT

Bactident Staph plus

Latex agglutination test kit for the rapid identification of Staphylococcus aureus from culture (for laboratory use only).
Cat. No. 1.13316.0001 (Pack with 50 tests)

Bactident Oxidase

Test strip with a reactive zone for detecting cytochrome oxidase in micro-organisms.
Cat No. 1.1220.0001 (50 test strips)

INDOLE

KOVAC's Indole Reagent

Reagent proposed by KOVACS (1928) for detecting microbial indole in the identification of indole-positive and indole-negative micro-organisms.
Cat No. 1.09293.0100 (100ml)

Bactident Indole

KOVACS indole reagent in a practical dropper bottle.
Cat No. 1.11350.0001 (30ml)

top

NITRITE REAGENT

GRIESS-ILOSVAY's Nitrite Reagent

Reagent solution for detecting microbial nitrite in order to identify nitrate-reducing micro-organisms.
Cat. No. 1.09023.0500 (500ml)

UROTEST

Urotest AB

Test system for the detection of antimicrobial substances (inhibitors) in urine.
Cat. No. 1.13194.0001 (Pack containing 50 tests)

LITMUS SOLUTION

Kubel Tiemann's Litmus Solution

The solution is used as a pH indicator particularly in the bacteriological examination of milk.
Cat. No. 1.05314.2500 (2.5l)

 top

Stains & Consumable Apparatus

Medical microbiology is concerned with the aetiology, pathogenesis (mode of infection), laboratory diagnosis and treatment of infection in the individual and with the epidemiology (study of mass disease) and control of infection in the community.

Microscopial examination is usually the first step taken for the identification of an unknown bacterium. The baterium may be allocated to one or other of the major groups when its morphology and staining reactions have been observed. The morphological features of importance are the size, shape and grouping of the cells and their possession of any distinctive structures such as endospores, flagella, capsules and intracellular granules.

Staining reactions are observed after treatment by procedures such as Gram and Ziehl-Neelsen stains, the different kinds of bacteria being shown in separate colours due to their different permeability to certain decolourising agents.

The microscopial examination of fixed and stained preparations is usually an essential routine procedure. The bacteria are more readily discovered and studied when immobilised by fixation and darkly stained in contrast with the bright background.

Simple staining is effected by the application of a watery solution of a single basic dye, eg Methylene Blue, Methyl Violet or Basic Fuchsin.

The coloured, positively charged cation of the basic dye combines firmly with negatively charged groups in the bacterial protoplasm, especially with those of the abundant nucleic acids. The stain is obtained through a subsequent washing with a water for the removal of excess dye from the slide. Acidic dyes, having coloured anions, do not stain bacteria strongly except at very acid pH values and thus can be used for negative staining.

Gram reactivity appears to reflect a fundamental aspect of cell structure and is correlated with many other biological properties. Thus, the different species of a single genus generally show the same reaction. Gram-positive bacteria are more susceptible than Gram-negative bacteria to antibacterial actions of penicillin, acids, iodine, basic dyes, detergents and lysozyme, and less susceptible to alkalis, azide, proteolytic enzymes, lysis by antibody and complement and plasmolysis in solutions of high osmotic pressure.

Gram Colour Staining Kit

The Gram staining procedure utilises aniline dyes, which are bound to the wall of the bacteria, converted to a coloured iodine complex. With this staining procedure bacteria can be distinguished as Gram-positive and Gram-negative. The coloured iodine complex in the cell wall of Gram-positive bacteria cannot be removed by a decolouring agent such as acetone or alcohol. The cell remains blue-violet. The coloured iodine complex of Gram-negative bacteria is removed by a decolourant and the cell is stained pink to red or orange by counterstaining.

Tb-colour Staining Kit

The Tb-colour staining kit can be used for microscopial examination of mycobacteria. The modified Ziehl-Neelsen staining is superior to the conventional method due to a carbol fuchsin solution, which works as a cold stain (no heating required). Development and inhalation of dangerous phenol vapours are therefore avoided. The brilliant red stained bacilli are easily distinguished for the green background.

For more information on microbiology stains and apparatus from Advanced please email sales@advtechind.com  

 top