100 assays. Diminished glutathione (GSH) level occurs at the early stages of apoptosis. The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes. The assay utilizes a dye th
Application
100 assays. Diminished glutathione (GSH) level occurs at the early stages of apoptosis. The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes. The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue (excitation = 380 nm; emission = 461 nm) when bound to glutathione. Thus the decrease in glutathione level in apoptotic cells can be easily detected using a fluorometer or a 96-well fluorometric plate reader.